Loading and Processing Contigs

Functions that load, combine, and process the single-cell contig information.

addVariable()

Adding Variables After combineTCR() or combineBCR()

combineBCR()

Combine B Cell Receptor Contig Data

combineTCR()

Combine T Cell Receptor Contig Data

createHTOContigList()

Deconvolute Contig Information from Multiplexed Experiments

getContigDoublets()

Get Contig Doublets

exportClones()

Export Clonal Data in Various Formats

loadContigs()

Load Immune Receptor Sequencing Contigs

subsetClones()

Subset The Product of combineTCR() or combineBCR()

annotateInvariant()

Annotate invariant T cells (MAIT or iNKT) in single-cell TCR data

Visualizing Clones

Functions plotting clonal data.

clonalAbundance()

Plot the Relative Abundance of Clones

clonalCluster()

Cluster clones by sequence similarity

clonalCompare()

Compare Clonal Abundance Across Variables

clonalDiversity()

Calculate Clonal Diversity

clonalHomeostasis()

Plot Clonal Homeostasis of the Repertoire

clonalLength()

Plot the Distribution of Sequence Lengths

clonalOverlap()

Examining the clonal overlap between groups or samples

clonalProportion()

Plot the Clonal Space Occupied by Specific Clones

clonalQuant()

Plot Number or Proportions of Clones

clonalRarefaction()

Calculate rarefaction based on the abundance of clones

clonalScatter()

Scatter Plot Comparing Clones Across Two Samples

clonalSizeDistribution()

Plot powerTCR Clustering Based on Clonal Size

percentGeneUsage() vizGenes() percentGenes() percentVJ()

Summarizes and Visualizes Gene Usage

Summarizing Repertoire

Functions to summarize clonal sequences across the repertoire.

percentAA()

Plot Relative Amino Acid Composition by Position

positionalEntropy()

Examining the Diversity of Amino Acids by Position

positionalProperty()

Plot Positional Physicochemical Property Analysis

percentGeneUsage() vizGenes() percentGenes() percentVJ()

Summarizes and Visualizes Gene Usage

percentKmer()

Analyze K-mer Motif Composition

Single-Cell Object Processing and Visualizations

Functions to add or visualize clonal information along a single-cell object.

alluvialClones()

Alluvial Plotting for Single-Cell Object

clonalBias()

Calculate Clonal Bias Towards a Cluster or Compartment

clonalNetwork()

Visualize Clonal Network in Dimensional Reductions

clonalOccupy()

Plot cloneSize by Variable in Single-Cell Objects

clonalOverlay()

Visualize Distribution of Clonal Frequency

combineExpression()

Adding Clonal Information to Single-Cell Object

getCirclize()

Generate Data Frame to Plot Cord Diagram

highlightClones()

Highlighting Specific Clones

quietVDJgenes() quietTCRgenes() quietBCRgenes()

Remove TCR and BCR genes from variable gene results

StartracDiversity()

Calculate Startrac-based Diversity Indices

Data

Reference data for package functions.

contig_list

A List of Eight Single-cell TCR Sequencing Runs.

scRep_example

A Seurat Object of 500 Single T cells,