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Loading and Processing Contigs

Functions that load, combine, and process the single-cell contig information.

loadContigs()
Load Immune Receptor Sequencing Contigs
combineTCR()
Combine T Cell Receptor Contig Data
combineBCR()
Combine B Cell Receptor Contig Data
clonalBin()
Bin Clones by Frequency or Proportion
addVariable()
Adding Variables After combineTCR() or combineBCR()
subsetClones()
Subset The Product of combineTCR() or combineBCR()
exportClones()
Export Clonal Data in Various Formats
createHTOContigList()
Deconvolute Contig Information from Multiplexed Experiments
getContigDoublets() experimental
Get Contig Doublets
annotateInvariant()
Annotate invariant T cells (MAIT or iNKT) in single-cell TCR data

Visualizing Clones

Functions for visualizing clonal data across samples and groups.

clonalAbundance()
Plot the Relative Abundance of Clones
clonalCompare()
Compare Clonal Abundance Across Variables
clonalDiversity()
Calculate Clonal Diversity
clonalHomeostasis()
Plot Clonal Homeostasis of the Repertoire
clonalLength()
Plot the Distribution of Sequence Lengths
clonalOverlap()
Examining the clonal overlap between groups or samples
clonalProportion()
Plot the Clonal Space Occupied by Specific Clones
clonalQuant()
Plot Number or Proportions of Clones
clonalRarefaction()
Calculate rarefaction based on the abundance of clones
clonalScatter()
Scatter Plot Comparing Clones Across Two Samples
clonalSizeDistribution()
Plot powerTCR Clustering Based on Clonal Size
percentGeneUsage() vizGenes() percentGenes() percentVJ()
Summarizes and Visualizes Gene Usage

Summarizing Repertoire

Functions to summarize clonal sequences across the repertoire.

percentGeneUsage() vizGenes() percentGenes() percentVJ()
Summarizes and Visualizes Gene Usage
percentAA()
Plot Relative Amino Acid Composition by Position
percentKmer()
Analyze K-mer Motif Composition
positionalEntropy()
Examining the Diversity of Amino Acids by Position
positionalProperty()
Plot Positional Physicochemical Property Analysis

Clustering and Similarity

Functions for clustering clones by sequence similarity.

clonalCluster()
Cluster clones by sequence similarity

Single-Cell Object Visualizations

Functions to add or visualize clonal information along a single-cell object.

combineExpression()
Adding Clonal Information to Single-Cell Object
alluvialClones()
Alluvial Plotting for Single-Cell Object
clonalBias()
Calculate Clonal Bias Towards a Cluster or Compartment
clonalNetwork()
Visualize Clonal Network in Dimensional Reductions
clonalOccupy()
Plot cloneSize by Variable in Single-Cell Objects
clonalOverlay()
Visualize Distribution of Clonal Frequency
getCirclize()
Generate Data Frame to Plot Chord Diagram
vizCirclize()
Visualize Clonal Relationships as a Chord Diagram
highlightClones()
Highlighting Specific Clones
StartracDiversity()
Calculate Startrac-based Diversity Indices

Utilities

Helper functions for filtering gene signatures and converting data.

quietVDJgenes() quietTCRgenes() quietBCRgenes()
Remove TCR and BCR genes from variable gene results
expression2List() deprecated
DEPRECATED Take the meta data in seurat/SCE and place it into a list

Data

Reference data for package functions.

contig_list
A List of Eight Single-cell TCR Sequencing Runs.
scRep_example
A Seurat Object of 500 Single T cells,