Immune Response

Leveraging single-cell transcriptomic data to uncover immune suppressive cancer cell subsets in triple-negative canine breast cancers
Single-cell RNA sequencing (scRNA-seq) has become an essential tool for uncovering the complexities of various physiological and …
Myung-Chul Kim, Myung-Chul Kim, Nick Borcherding, Woo-Jin Song,, Ryan Kolb, Weizhou Zhang
PDF DOI Pubmed
CD4+ T cells exhibit distinct transcriptional phenotypes in the lymph nodes and blood following mRNA vaccination in humans
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and mRNA vaccination induce robust CD4+ T cell responses. Using …
Nick Borcherding, Wooseob Kim, Michael Quinn, Fangjie Han, Julian Q Zhou, Alexandria J Sturtz, Aaron J Schmitz, Tingting Lei, Stefan A Schattgen, Michael K Klebert, Teresa Suessen, William D Middleton, Charles W Goss, Chang Liu, Jeremy Chase Crawford, Paul G Thomas, Sharlene A Teefey, Rachel M Presti, Jane A O'Halloran, Jackson S Turner, Ali H Ellebedy, Philip A Mudd
PDF Cite Code DOI Pubmed
CD4+ T cells exhibit distinct transcriptional phenotypes in the lymph nodes and blood following mRNA vaccination in humans
Single-cell immune repertoire analysis
Single-cell T cell and B cell antigen receptor-sequencing data analysis can potentially perform in-depth assessments of adaptive immune …
Sergio Irac, Megan Sioe Fei Soon, Nick Borcherding, Zewen Kelvin Tuong
PDF Cite DOI Pubmed
Type I Interferon Drives a Cellular State Inert to TCR‐Stimulation and Could Impede Effective T‐Cell Differentiation in Cancer
Head and neck squamous cell carcinoma (HNSCC) is linked to human papillomavirus (HPV) infection. HPV-positive and HPV-negative HNSCC …
Dillon Corvino, Martin Batstone, Brett G M Hughes, Tim Kempchen, Susanna S Ng, Nazhifah Salim, Franziska Schneppenheim, Denise Rommel, Ananthi Kumar, Sally Pearson, Jason Madore, Lambross T Koufariotis, Lisa Maria Steinheuer, Dilan Pathirana, Kevin Thurley, Michael Hölzel, Nick Borcherding, Matthias Braun, Tobias Bald
PDF Cite DOI Pubmed
T Cell Clonal Analysis Using Single-cell RNA Sequencing and Reference Maps
T cells are endowed with T-cell antigen receptors (TCR) that give them the capacity to recognize specific antigens and mount …
Massimo Andreatta, Paul Gueguen, Nick Borcherding, Santiago Carmona
PDF Cite DOI Pubmed
Multifunctional cytokine production marks influenza A virus-specific CD4 T cells with high expression of survival molecules
Cytokine production by memory T cells is a key mechanism of T cell mediated protection. However, we have limited understanding of the …
Lotus Westerhof, Jonathan Noonan, Kerrie Hargrave, Elizabeth Chimbayo, Zhiling Cheng, Varintra Lander, Thomas Purnell, Mark Jackson, Nick Borcherding, Megan MacLeod
PDF Cite DOI Pubmed
Tumor-associated fibrosis impairs immune surveillance and response to immune checkpoint blockade in non-small cell lung cancer
Non–small cell lung cancer (NSCLC) is a leading cause of cancer-related deaths. Immune checkpoint blockade has improved survival for …
Brett H Herzog, John M Baer, Nick Borcherding, Natalie L Kingston, Jad I Belle, Brett L Knolhoff, Graham D Hogg, Faiz Ahmad, Liang-I Kang, Jessica Petrone, Chieh-Yu Lin, Ramaswamy Govindan, David G DeNardo
PDF Cite DOI Pubmed
IL-17 cytokines preferentially act on naïve CD4+ T cells with the IL-17AF heterodimer inducing the greatest functional changes.
CD4+ T-helper 17 (Th17) T cells are a key population in protective immunity during infection and in self-tolerance/autoimmunity. …
Michael P Crawford, Nick Borcherding, Nitin J Karandikar
PDF Cite DOI Pubmed
IL-17 Producing Lymphocytes Cause Dry Eye and Corneal Disease With Aging in RXRα Mutant Mouse
Jehan Alam, Ghasem Yazdanpanah, Rinki Ratnapriya, Nick Borcherding, Cintia S De Paiva, DeQuan Li, Rodrigo Guimaraes de Souza, Zhiyuan Yu, Stephen C Pflugfelder
PDF Cite DOI Pubmed
Dissecting the cellular landscape and transcriptome network in viral myocarditis by single-cell RNA sequencing
Coxsackievirus B3 (CVB3)-induced myocarditis is commonly employed to study viral pathogenesis in mice. Chronically affected mice may …
Ninaad Lasrado, Nick Borcherding, Rajkumar Arumugam, Timothy K Starr, Jay Reddy
PDF Cite Code DOI Pubmed